Cumulative CAMAG
Bibliography Service CCBS

PLoS One 20(5), e0324390 (2025).
Samples were polar lipids extracted from Cupriavidus consociatus and C. oxalaticus (Burkholderiaceae) through the Bligh and Dyer’s technique. Two-dimensional TLC on silica gel with chloroform – methanol – water 14:6:1, followed by chloroform – methanol – acetic acid 13:5:2. Derivatization by spraying with ninhydrine for amino-lipids (phosphatidylethanolamine was detected in all strains), or with periodate Schiff’s reagent for glycolipids (phosphatidylglycerol was detected in C. consociatus, and cardiolipin was detected in both species).

Food Chem. 493, 145746 (2025). HPTLC of lipids (free fatty acids, acylglycerols, sterols, sterol esters, glycolipids, phospholipids, tocopherol), diterpenes (cafestol and kahweol), and alkaloids (caffeine and theobromine) in coffee leaves on silica gel with chloroform - methanol - water 34:8:1 for polar compounds; chloroform - methanol - water 180:20:3 for alkaloids; and hexane - diethyl ether - acetic acid 75:25:1 for non-polar compounds. Detection by spraying with cupper sulfate - phosphoric acid - methanol - water 10:8:5:78 or thymol or ninhydrin reagent. Qualitative identification under UV light at 254 and 366 nm. 

PLoS One 20(5), e0324608 (2025).
TLC on silica gel 1) to monitor the synthesis of dinitrobenzoic acid derivatives, and 2) to investigate their antimycobacterial mechanism. For 1) method A) with dichloromethane – methanol 93:7 for methyl 3,5-dinitrobenzoate (hRF 70), its hydrazide (hRF 30), and its semicarbazides = hydrazine-carboxamides (hRF 40-56) and thiosemicarbazide = hydrazine-carbothioamide (hRF 36); B) with dichloromethane – methanol 97:3 for oxadiazolamide derivatives (hRF 29-70) and for thiadiazolamide derivative (hRF 84); C) with pure chloroform or with n-hexane – ethyl acetate 1:1 for oxadiazolamines and thiadiazolamines (hRF 17-79). Detection under UV 254 nm.  For 2) lipids were isolated from Mycobacterium tuberculosis (Mycobacteriaceae) after 24h culture with radioactive 14C-acetate, in a growth medium untreated or treated (four of the synthetized derivatives were tested). TLC separation with chloroform – methanol – water 40:8:1. Visualization through autoradiography in an automated imager. In treated cells, trehalose monomycolates and dimycolates were accumulated, whereas phosphatidylethanolamine (PEA) was slightly increased and cardiolipin was unchanged. Therefore, the proposed mechanism was an inhibition of epimerase DprE1.

Phytochem. Anal. 36, 7-29 (2025). Review of the use of DNA barcoding and alternative/complementary testing in plant-related sectors to enhance quality assurance and accurate identification. The paper described HPTLC fingerprinting as effective technique in ensuring the quality and safety of herbal products and reviewed orthogonal approaches for authenticating herbal medicinal products.

J. Food Compos. Anal. 136, 106855 (2024). HPTLC of anthocyanins in the fruits of some cultivated varieties of Rubus occidentalis (black raspberries) and Rubus idaeus (red raspberries) and their hybrids on silica gel with ethyl acetate - water - formic acid 10:3:3. Quantitative determination by absorbance measurement at 520 nm. The hRF value for anthocyanin was 36. Linearity was between 364 and 2000 ng/zone. The inter-day and intra-day precision was below 5 % (n=9). LOD and LOQ were 101 and 364 ng/zone, respectively.